FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_60.34100000009c9c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_60.34100000009c9c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847591
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC11790.1391001084249361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG1750.034.71301318
TATGCCG1900.033.15667743
CCCACGA2000.031.49884419
ACCCTGG2050.030.73057727
GCGCATT300.00514742829.998915
GAGACCC2100.029.99889824
GGTGCGC406.166122E-428.1239667
CGAGACC2250.027.99897223
CCGAGCC2250.026.99900814
ATGCCGT2350.026.80752644
CGTATGC2400.026.24903741
TGCCGTC2500.026.09904145
CGAGCCC2500.025.19907415
CCCTGGA2500.025.19907428
GCGGCCC450.001226551224.99908319
ACGAGAC2550.024.70497522
AGACCCT2700.024.16577725
CTCGTAT2650.023.7727139
GACCCTG2900.023.2750126
GTATGCC2750.022.9082542
Produced by FastQC (version 0.11.2)