FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_6.34100000009883.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_6.34100000009883.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797797
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC11820.14815799006514188RNA PCR Primer, Index 46 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2250.032.9990843
TGCCGTC2450.031.22362145
CCCACGA2400.030.93664219
ATGCCGT2450.030.3052844
CGAGACC2400.029.99916623
GCCCACG2500.028.79919418
CGTATGC2600.028.55689641
GTATGCC2700.027.49923342
ACGAGAC3100.025.4025222
TCGTATG2900.024.82689540
CACGAGA3000.024.74931121
TTCCGAA3050.024.34358431
CTCCGAG3000.023.99933212
CGAGCCC3000.023.99933215
CCGTCGG851.8926403E-723.82286813
CCGAGCC3100.023.2251614
TCCGAAT3200.023.20247832
ATTCCGA3300.022.49937430
CGAATCT3300.022.49937434
CGGCCGT702.7022365E-522.49937210
Produced by FastQC (version 0.11.2)