FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_59.34100000009c8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_59.34100000009c8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209237
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC55160.45615541039515No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT12160.100559278288706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7050.039.89204445
GGGGCTC250.00210756135.9985775
ATGCCGT8050.034.9365144
TATGCCG8900.031.59987443
CGTATGC9000.030.99877741
GTATGCC9200.030.3248942
TATCTCG9650.028.67762436
TCGCCCC1100.028.63523325
CGCCCCT1100.028.63523326
GCCCACG9700.028.52980218
CCCACGA9800.028.2386819
TCGTATG10000.027.89889740
CCACGAG9950.027.81297120
CTCGTAT10050.027.76009839
ATAATAT9900.027.72617532
TACCGCG1550.027.5795567
CGAGACA10100.027.39990823
ACCGCGA1650.027.271658
CATAATA10100.026.95438231
CACGAGA10300.026.8678721
Produced by FastQC (version 0.11.2)