FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_58.34100000009c72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_58.34100000009c72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215251
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC86530.7120339748743263No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT13000.10697378566238579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9700.041.05497445
ATGCCGT11250.035.3985144
TATGCCG11900.033.46497743
GTATGCC12200.033.01090642
GCCCACG12500.032.21864318
CGTATGC12500.032.0386541
CCCACGA12800.031.46351819
GACTTGC12800.030.93619726
CGAGACT12950.030.9253423
ACGAGAC13150.030.79718422
AGCCCAC13350.030.1672717
TCGTATG13350.030.1672740
CTGCGCG300.005148431329.9987379
CTCGTAT13250.029.88553639
CGAGCCC13900.028.97360215
TCTCGTA13700.028.90389438
CCACGAG14050.028.66427420
CCGAGCC14400.028.12381714
GCGCGCT406.1678677E-428.12381611
AATCTCG13950.028.06333736
Produced by FastQC (version 0.11.2)