FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_56.34100000009c59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_56.34100000009c59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910484
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC77260.8485596671660347No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT13320.1462958162911155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8850.040.67642645
ATGCCGT9850.036.5468444
GCCCACG10900.032.8198618
CAAGGCG10950.032.46452329
TATGCCG11200.032.34252543
CCCACGA11300.031.857219
GTATGCC11450.031.63635842
CCACGAG11450.031.63635820
AGCCCAC11350.031.51863117
CCGAGCC11400.031.38039214
CGAGCCC11550.031.1676515
TCGTATG11550.030.97285340
GCGAATC11350.030.9239433
GGCGAAT11350.030.9239432
CTCGTAT11600.030.64539139
AGGCGAA11550.030.58325831
GAGCCCA11850.030.37859516
ACGAGAC12100.030.12282822
CACGAGA12100.029.93688421
CGTATGC12050.029.87438841
Produced by FastQC (version 0.11.2)