H23V3BCXX l02n01 kb2_1_55.34100000009c4c.fastq.gz FastQC Report

Summary

Basic Statistics
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Adapter Content
Kmer Content

Basic Statistics

Measure	Value
Filename	H23V3BCXX l02n01 kb2_1_55.34100000009c4c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1431
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	33	2.3060796645702304	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	17	1.187980433263452	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	13	0.9084556254367574	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT	10	0.6988120195667366	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	10	0.6988120195667366	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	3	0.20964360587002098	RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT	2	0.1397624039133473	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	2	0.1397624039133473	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT	2	0.1397624039133473	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG	2	0.1397624039133473	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCCG	20	4.885333E-4	45.0	10
CACATCT	20	4.885333E-4	45.0	7
TATACAC	20	4.885333E-4	45.0	3
CATCTCC	20	4.885333E-4	45.0	9
ACATCTC	20	4.885333E-4	45.0	8
TTATACA	20	4.885333E-4	45.0	2
TACACAT	20	4.885333E-4	45.0	5
ACACATC	20	4.885333E-4	45.0	6
CTTATAC	20	4.885333E-4	45.0	1
CCGAGCC	20	4.885333E-4	45.0	14
TCTCCGA	20	4.885333E-4	45.0	11
TCCGAGC	20	4.885333E-4	45.0	13
ATACACA	20	4.885333E-4	45.0	4
CTCCGAG	20	4.885333E-4	45.0	12