FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_53.34100000009c22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_53.34100000009c22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193775
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC56020.4692676593160353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6100.038.35908545
ATGCCGT7050.033.1901344
TATGCCG7600.030.49217243
CGACCTA7550.029.80010631
TATCTCG7650.029.70466436
CGTATGC8000.029.24880441
GCGACCT7800.029.1334230
GCCCACG8000.028.96756618
CCCACGA8050.028.78764219
CTGCGAC8050.028.22865928
ACCTATC8050.028.22865933
TCGTATG8300.028.19161440
TGCGACC8100.028.05440729
CGAGCCC8350.028.02280415
GACTGCG8050.027.94916726
CCGAGCC8450.027.42491314
GACCTAT8300.027.10732332
CTCGTAT8600.026.94657139
CCTATCT8500.026.734234
ACTGCGA8550.026.57785827
Produced by FastQC (version 0.11.2)