FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_52.34100000009c15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_52.34100000009c15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267103
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC139751.1029095503680442No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT21810.1721249180216604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC16150.042.07286545
TCGCGCG301.1402173E-437.498729
ATGCCGT18300.037.0069344
TATGCCG19000.035.76193243
GCCCACG19200.035.27223218
CGTATGC19700.034.6054141
CCCACGA19750.034.28996719
GTATGCC19950.034.05898742
TCGTATG20050.033.88911440
AGCCCAC20100.033.6928817
CTCGTAT20250.033.55440539
ACGAGAC20400.033.52826722
CGAGACC20150.033.16263623
CCACGAG20500.033.03545820
CACGAGA21500.031.91751321
CCGAGCC21350.031.82561114
CCTTGCA21050.031.74475928
CGAGCCC21400.031.54097715
ACCTTGC21150.031.48828727
TCTCGTA21600.031.45725638
Produced by FastQC (version 0.11.2)