FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_51.34100000009c08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_51.34100000009c08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889200
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC65910.7412280701754386No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA21270.2392037786774629No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA21040.23661718398560505No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG16880.1898335582546109No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG16350.18387314439946018No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG15410.17330184435447593No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT11390.12809266756635176No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG10120.11381016644174538No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG10010.11257309941520469No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC9360.10526315789473684No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG9040.10166441745389114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7050.040.53044545
ATGCCGT8300.034.42646444
CTAACGC2300.034.2378923
TATGCCG8950.031.92621643
GCCCACG8900.031.85277718
GTATGCC9050.031.57344242
CTCGTAT9100.031.15271839
CCCACGA9250.030.8907719
AGACTAG9300.030.72469525
TATCTCG9200.030.56954636
TCGTATG9350.030.56039240
CGTATGC9500.030.07785641
CGAGACT9650.029.84347723
GCTAACG2650.029.7159042
AGCCCAC9600.029.5301817
CGAGCCC9600.029.5301815
CCTATCT9400.029.4404234
ATCCTAT9500.029.36735532
CCACGAG9750.029.30663120
ACGAGAC9900.028.8625922