FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_50.34100000009bfa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_50.34100000009bfa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779093
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC18320.23514522656473616TruSeq Adapter, Index 12 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCA700.041.784467
TATGCCG2650.035.6593143
CGTATGC2800.035.35608341
CACGAGA2800.035.35608321
ATGCCGT2750.034.36260644
GCCCACG2800.033.74898518
CCCACGA2900.032.58522819
CGAGACT2900.032.58522823
ACGAGAC3050.032.45804222
AGCCCAC3050.031.7203617
CCACGAG3000.031.49905620
TGCCGTC3000.031.49905645
GTATGCC3100.030.48295842
CCGAGCC3100.030.48295814
CTCGTAT3200.030.23346939
TCCGAGC3300.029.31730313
CGCAAGG1001.8189894E-1229.24912310
TCGTATG3400.029.11677440
GGGCTGA554.1636413E-628.6355066
CTCCGAG3300.028.63550412
Produced by FastQC (version 0.11.2)