FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_5.34100000009869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_5.34100000009869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645135
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA68171.05667805963093No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT62520.9690994908042502No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA49020.7598409635192634No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG36150.5603478341742426No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT34730.538336937230192No Hit
GTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCT21210.3287683973121905No Hit
ACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAA20680.3205530625372984No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC17320.2684709401908128No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTGTCTCTTATAC15550.2410348221690034No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGACTGTCTCT14560.225689196834771No Hit
GTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGCTGTCTCTTATAC14210.2202639757570121No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAA14060.21793888100940115No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTACTGTCTCT12930.20042316724406517No Hit
AGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGCTGTCTCTTAT11060.17143698605718183No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGACTGTCTC10060.15593635440644207No Hit
GTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTT10020.1553163291404125No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAG9650.14958109542963877No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCTGTCTCTTAT9450.14648096909949082No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACTGTCTC9220.14291582381982065No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTC7850.12167995845830717No Hit
GCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTACTG7670.11888984476117401No Hit
ATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTC7480.11594472474753345No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG7290.11299960473389291No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCCTG7250.11237957946786331No Hit
ATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTACTGTCTC7160.11098452261929673No Hit
AAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAAACC6980.10819440892216357No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATCTGTCTC6890.10679935207359699No Hit
CCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAAACCTGTCTCTT6640.10292419416091204No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATCTGTCTC6490.1005990994133011No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2200.037.839844
TCCTAGG301.13956296E-437.4989052
CTCGCAA250.00210672835.99894310
CCGTTAC6200.034.883651
TGCGACC2350.034.4670829
TATGCCG2400.033.74901243
CCCACGA2500.033.29902619
GACTGCG2500.033.29902626
CTGCGAC2450.033.06025728
GTATGCC2450.033.06025742
AGACTGC2500.032.3990525
GCCCACG2550.031.76377518
GAGTTTA13800.030.854971
GTTCGTA2900.030.2976551
TGCCGTC2750.030.27184145
AACAACG300.0051463429.9991237
TATCTCG2700.029.99912336
CGCAAAC300.0051463429.99912312
CCACGTT300.0051463429.99912341
AGTTTAA14550.029.2259482
Produced by FastQC (version 0.11.2)