FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_49.34100000009bd0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_49.34100000009bd0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492559
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG11250.22839903443039308No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC8050.1634321979701924No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA6360.1291215874646489No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC5930.12039166881530944No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGC5930.12039166881530944No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT5770.1171433269922994No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA5470.11105268607415558No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA5290.10739830152326929No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT5200.10557110924782616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAC300.005145514429.9987144
ACCTATA2300.029.4032761
ATACCCG2350.028.7221765
GTCCTAG406.09344E-428.178141
TACCCGG2450.027.5498396
GGGGCAA2400.027.18633518
CGTATGC755.6277713E-826.99884241
TCGCGCG2800.026.516729
CGCGCGC2950.025.16841110
AGGCCTC2700.024.99892830
GCCTCGA2700.024.99892832
ATGCCGT901.187982E-824.99892844
GGTATCA3400.024.5315591
CCCACGA651.5219599E-524.2297319
TATCTCG751.6949962E-623.99897236
GTCGCGC3100.023.9505868
TATGCCG851.8898572E-723.82250843
CGTCGGG2650.023.77256614
GCGCGCT4200.023.57041711
TAGTTGA3200.023.20213141
Produced by FastQC (version 0.11.2)