FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_48.34100000009bc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_48.34100000009bc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398108
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC12960.3255398032694646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.14594029760768434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2150.035.58090243
CGTATGC2250.033.99952741
ATGCCGT2300.033.26040644
GTATGCC2400.031.87455742
GCCCACG2550.031.76426118
TCGTATG2450.031.22405240
CTCGTAT2500.030.59957139
TGCCGTC2650.030.5656145
CCCACGA2600.030.28803819
ACGAGAC2650.029.71656622
TCTCGTA2600.029.42266738
CGAGACC2100.028.9281723
CTCCGAG2850.028.42065412
CACGAGA2950.027.45724321
CCGAGCC2900.027.15479314
CCTGGAT2250.026.99962429
GAGACCC2350.026.80813824
ACCCTGG2400.026.24963627
CTGGATA2350.025.85070430
AGACCCT2450.025.71392625
Produced by FastQC (version 0.11.2)