FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_47.34100000009bb6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_47.34100000009bb6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389353
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC83010.5974723486399784No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT23620.17000719039725684No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA16250.11696091634019576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10200.039.70477345
GTCGCGC401.5611002E-533.7490548
TATGCCG12550.032.4492943
GTATGCC12850.031.8668142
CGTATGC12900.031.74329841
ATGCCGT12850.031.6917244
CCCACGA12900.031.22005719
GCCCACG13000.030.80683118
TCGTATG13250.030.56518240
ACGAGAC13400.030.55884622
CCACGAG13250.030.39537420
GGTCGCG453.492031E-529.9991597
CGAGACA12750.029.11683323
CGACGCT1250.028.79919228
TATCTCG13850.028.75370836
CACGAGA14150.028.62110721
CTCGTAT14300.028.47822439
TCTCGTA14250.028.42025838
CGCTATA1300.027.69153231
GACGCTA1300.027.69153229
Produced by FastQC (version 0.11.2)