FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_46.34100000009baa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_46.34100000009baa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254269
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC327772.613235278875584No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT104250.8311614175268622No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC24630.1963693593639004No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC22380.17843062373382423No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC20360.1623256255237114No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC18100.1443071621797238No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG17210.13721139564160478No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA16590.1322682773790949No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA16170.12891971339481403No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA13000.10364602808488449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC35550.043.47958845
GTCCGGC750.039.056331
CGGCATG950.033.1568074
ATGCCGT47100.032.81739844
CGTATGC48800.031.62806141
TATGCCG49000.031.59080143
GTATGCC49450.031.3488242
TCGTATG49250.031.33907340
CCACGAG49200.031.2794620
ACGAGAC49700.031.1911322
CTCGTAT49650.031.08659439
TCTCGTA49650.031.08659438
CACGAGA49800.030.99296221
CGAGACT49850.030.96187223
TGCTGGA50200.030.74600430
CCCACGA50000.030.73399419
GCTGGAA50150.030.6869331
GACTTGC50300.030.64014826
ATCTCGT50750.030.45713237
TTGCTGG50850.030.4414829