FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_45.34100000009b9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_45.34100000009b9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210285
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC871557.201196412415258No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT118800.981586981578719No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA90850.7506496403739615No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA86980.7186737008225336No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG67400.5568936242290039No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG64590.5336759523583289No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG50400.4164308406697596No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG49490.40891195049100004No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC43250.35735384640807744No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC40950.33835005804417967No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG36670.30298648665396993No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG34770.2872877049620544No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG34300.2834043221224753No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC33910.2801819406172926No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC32980.27249780010493396No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT32040.2647310344257758No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT30320.2505195057362522No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT29620.2447357440602833No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT29470.24349636655829002No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA28100.23217671870675088No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT27620.22821071070037222No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT26480.2187914416852229No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA26310.21738681384963046No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG24670.20383628649450336No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG23640.19532589431414915No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCC22520.18607187563259894No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC21650.1788834861210376No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC21270.1757437297826545No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA20670.17078621977468117No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC20480.16921634160548962No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA20470.16913371643869005No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA20100.1660765852671065No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC19670.1625237030947256No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA19320.15963182225674116No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC18750.15492218774916652No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA17980.14856004990560076No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA17830.1473206724036074No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCC17750.14665967106921096No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC17130.14153691072763852No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC16990.14038015839244475No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCTC16720.13814927888885675No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTC16060.1326960178800861No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC16040.132530767546487No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC15930.13162189071169186No Hit
CGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC15890.13129139004449364No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATC15610.1289778853741061No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA15530.12831688403970967No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTC15510.12815163370611055No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA15400.12724275687131542No Hit
CGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT15030.12418562569973188No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT14760.12195474619614388No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCTGTCTCTTATACACATCTC14560.12030224286015277No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACAT13980.11550998318577854No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT13740.11352697918258924No Hit
GTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC13570.11212235134699679No Hit
TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA13040.10774321750662035No Hit
ATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG12910.10666909033822612No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTCC12530.10352933399984302No Hit
TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA12230.10105057899585634No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCC12180.10063745316185857No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC93300.044.15461345
ATGCCGT105400.039.12832344
TATCTCG108850.037.8674836
GCTATAT109300.037.75274732
GACGCTA109500.037.7454329
CGCTATA109250.037.72883631
GAGACGA110150.037.70652824
ACGAGAC110200.037.68942322
AGACGAC110100.037.6827825
ACGACGC110100.037.6827827
ACGCTAT109650.037.6732830
GACGACG110100.037.66234626
TATGCCG109950.037.63187443
ATATCTC109500.037.62214735
CGAGACG110350.037.617823
CTATATC109600.037.60835333
CTCGTAT109800.037.6013239
CGACGCT110100.037.58060528
GTATGCC110300.037.51246342
CCACGAG110550.037.5090420
Produced by FastQC (version 0.11.2)