FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_44.34100000009b80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_44.34100000009b80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231544
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA2550.029.99864619
TGCCGTC2600.028.55640445
GCCCACG2750.027.81692518
CCGAGCC2800.027.32019414
ATGCCGT2850.026.05145844
TATGCCG2950.025.93103443
CGAGCCC3150.024.99887315
GTATGCC3300.024.54434842
AGCCCAC3150.024.28461817
CAAGGCG2350.023.93509129
TCGCGCG851.8946957E-723.8224569
CGAGACC2500.022.49898523
CGTATGC3550.022.18209641
GCGAATC2450.022.03982233
ACGAGAC3700.021.89090522
CTCCGAG3500.021.85615712
GTCGCGC955.5347846E-721.3148278
GAGCCCA3950.019.36621716
GGTCGCG1051.446364E-619.2848457
CACGAGA4200.019.28484521
Produced by FastQC (version 0.11.2)