FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_43.34100000009b73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_43.34100000009b73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC50623.523942352394236No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT462.1385402138540215No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC462.1385402138540215No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC100.46490004649000466No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT90.41841004184100417No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC80.3719200371920037No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTTC60.2789400278940028Illumina PCR Primer Index 11 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG50.23245002324500233No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT40.18596001859600186No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCAGTC30.1394700139470014No Hit
CTTATACAAATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC30.1394700139470014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC550.045.00000445
AGCCCAC759.094947E-1233.017
ATCTCGT759.094947E-1233.037
ATCTCCG759.094947E-1233.010
CACATCT759.094947E-1233.07
GCCCACG759.094947E-1233.018
CCACGAG759.094947E-1233.020
TATGCCG759.094947E-1233.043
GAGCCCA759.094947E-1233.016
CACGAGA759.094947E-1233.021
CCCACGA759.094947E-1233.019
ACATCTC759.094947E-1233.08
TCTCGTA759.094947E-1233.038
ACGAGAC759.094947E-1233.022
ATGCCGT759.094947E-1233.044
CGTATGC759.094947E-1233.041
CGAGCCC759.094947E-1233.015
CCGAGCC759.094947E-1233.014
TCGTATG759.094947E-1233.040
TCTCCGA759.094947E-1233.011
Produced by FastQC (version 0.11.2)