FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_42.34100000009b66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_42.34100000009b66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC17715.391304347826088No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC373.217391304347826No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT181.565217391304348No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC80.6956521739130435No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT80.6956521739130435No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC50.43478260869565216No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG40.34782608695652173No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC30.26086956521739135RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC30.26086956521739135No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT20.17391304347826086No Hit
TATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGAGC20.17391304347826086No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT20.17391304347826086Illumina PCR Primer Index 11 (95% over 21bp)
ATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGA20.17391304347826086No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG20.17391304347826086No Hit
CTTATACACATCTCCGAGCCCACGAGCCGACGCTATATCTCGTATGCCGTC20.17391304347826086No Hit
CTTATACACATCTCCGAGCCCCACGAGACGACGCTATATCTCGTATGCCGT20.17391304347826086No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTTC20.17391304347826086Illumina PCR Primer Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCC501.1823431E-1040.514
AGCCCAC409.230098E-839.37500417
GCCCACG409.230098E-839.37500418
GAGCCCA409.230098E-839.37500416
CCCACGA409.230098E-839.37500419
CGAGCCC409.230098E-839.37500415
CTATATC352.364579E-638.57142633
ACGCTAT352.364579E-638.57142630
TATATCT352.364579E-638.57142634
CGCTATA352.364579E-638.57142631
GCTATAT352.364579E-638.57142632
GACGCTA352.364579E-638.57142629
TATCTCG352.364579E-638.57142636
AGACGAC352.364579E-638.57142625
GACGACG352.364579E-638.57142626
CGACGCT352.364579E-638.57142628
ACGACGC352.364579E-638.57142627
CGAGACG352.364579E-638.57142623
ATATCTC352.364579E-638.57142635
GAGACGA352.364579E-638.57142624
Produced by FastQC (version 0.11.2)