FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_41.34100000009b5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_41.34100000009b5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507502
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC53930.35774413566283825No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT15330.10169140737458392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7450.038.65632245
GACTGCG8350.033.6814226
GCCCACG8850.033.5581118
TGCGACC8250.033.27152629
GCGACCT8150.033.12763630
TATGCCG8750.032.91309743
CTGCGAC8450.032.75029828
CGACCTA8300.032.2578731
ATGCCGT8950.032.1776144
CCCACGA9250.032.10694519
ACTGCGA9100.030.90547827
CCACGAG9650.030.7760920
AGCCCAC9950.030.30041317
CGAGACT9450.030.23700523
CGAGCCC9850.030.15119615
CGGGGGG453.4924196E-529.99891517
CGTATGC9850.029.4659441
GTATGCC9800.029.38669442
TATCTCG9750.029.30663336
CCGAGCC10250.028.97456614
Produced by FastQC (version 0.11.2)