FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_40.34100000009b4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_40.34100000009b4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693719
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC108720.6419010473402023No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT17580.10379525765490025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11550.041.29726845
ATGCCGT13650.035.273544
GCCCACG14850.033.18066418
TATGCCG14550.032.93699643
CCCACGA15300.032.35181419
TCGTATG15400.031.99564240
CGTATGC15550.031.25293441
GTATGCC15350.031.22041342
AGCCCAC15900.030.84798217
CTCGTAT15850.030.51944439
CGAGCCC16450.030.0901415
TGGCCGG300.005148815529.99895722
CCACGAG16500.029.99895720
CGAGACC16100.028.78781923
CCGAGCC17150.028.73077814
ACGAGAC17400.028.70589822
CACGAGA17350.028.65894721
ACCTTGC16250.028.38362727
TCTCGTA17050.028.37144938
CCTTGCA16550.028.00506828
Produced by FastQC (version 0.11.2)