FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_4.34100000009840.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_4.34100000009840.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440330
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTATAGAACTCTAACTGCTTGTAAGGCTTGTAATTCAAGATCTATTTC7580.17214361955805874No Hit
GTATAGTATATCTAGCGATAGTGAAGAGTAACGTGAGTGAAAGGTGAAAAG5200.11809324824563397No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC5090.11559512184043785No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4770.10832784502532193No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.10696523062248768No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4700.10673812822201531No Hit
GTATAGATTCTATCCTCGGAAGGAAACAAGAATAGAAGAAGAACCTCTAAT4490.10196897781209546No Hit
CTATAGAACTCTAACTGCTTGTAAGGCTTGTAATTCAAGATCTATTTCACC4470.10151477301115074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG207.029764E-444.9984121
CCTAGCG250.002106065435.9987267
TCCCACG250.002106065435.9987262
TGCTACG300.005144738629.9989417
TAGTGCC300.005144738629.9989419
ACGAGGA300.005144738629.99894112
AAATGCG300.005144738629.99894119
CCGGAAA406.1613275E-428.12400645
GTTATAT1450.026.4203831
TCGTATG957.385097E-1026.05171240
CTCGTAT801.05106665E-725.31160539
TATGCCG901.1872544E-824.99911743
TATATAA551.3666588E-424.5445862
GGGGCAA751.6942031E-623.99915118
CGTATGC851.888784E-723.82268741
TGCCGTC851.888784E-723.82268745
GACAGTA602.4647455E-422.4992077
GCCCACG903.2785283E-722.49920518
CCATAGC500.00226287322.4992053
GTAAGAC500.00226287322.4992053
Produced by FastQC (version 0.11.2)