##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_1_39.34100000009b30.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26900 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.51245353159851 38.0 38.0 38.0 32.0 38.0 2 36.744721189591075 38.0 38.0 38.0 32.0 38.0 3 36.645018587360596 38.0 38.0 38.0 32.0 38.0 4 36.76806691449814 38.0 38.0 38.0 32.0 38.0 5 36.83003717472119 38.0 38.0 38.0 32.0 38.0 6 38.74453531598513 40.0 38.0 40.0 38.0 40.0 7 38.57494423791822 40.0 38.0 40.0 38.0 40.0 8 38.577434944237915 40.0 38.0 40.0 38.0 40.0 9 38.592007434944236 40.0 38.0 40.0 38.0 40.0 10 38.553308550185875 40.0 38.0 40.0 38.0 40.0 11 38.59847583643123 40.0 38.0 40.0 38.0 40.0 12 38.425873605947956 40.0 38.0 40.0 38.0 40.0 13 38.53881040892193 40.0 38.0 40.0 38.0 40.0 14 38.54539033457249 40.0 38.0 40.0 38.0 40.0 15 38.520520446096654 40.0 38.0 40.0 38.0 40.0 16 38.42981412639405 40.0 38.0 40.0 38.0 40.0 17 38.36472118959108 40.0 38.0 40.0 38.0 40.0 18 38.38081784386617 40.0 38.0 40.0 38.0 40.0 19 38.38014869888476 40.0 38.0 40.0 38.0 40.0 20 38.401635687732345 40.0 38.0 40.0 38.0 40.0 21 38.430297397769515 40.0 38.0 40.0 38.0 40.0 22 38.49591078066914 40.0 38.0 40.0 38.0 40.0 23 38.45468401486989 40.0 38.0 40.0 38.0 40.0 24 38.43843866171004 40.0 38.0 40.0 38.0 40.0 25 38.399814126394055 40.0 38.0 40.0 38.0 40.0 26 38.384126394052046 40.0 38.0 40.0 38.0 40.0 27 38.32513011152416 40.0 38.0 40.0 38.0 40.0 28 38.346691449814124 40.0 38.0 40.0 38.0 40.0 29 38.269219330855016 40.0 38.0 40.0 38.0 40.0 30 38.32557620817844 40.0 38.0 40.0 38.0 40.0 31 38.333977695167285 40.0 38.0 40.0 38.0 40.0 32 38.31405204460967 40.0 38.0 40.0 38.0 40.0 33 38.32810408921933 40.0 38.0 40.0 38.0 40.0 34 38.33401486988848 40.0 38.0 40.0 38.0 40.0 35 38.28624535315985 40.0 38.0 40.0 38.0 40.0 36 38.24014869888476 40.0 38.0 40.0 38.0 40.0 37 38.16364312267658 40.0 38.0 40.0 38.0 40.0 38 38.22052044609666 40.0 38.0 40.0 38.0 40.0 39 38.10267657992565 40.0 38.0 40.0 38.0 40.0 40 38.16684014869888 40.0 38.0 40.0 38.0 40.0 41 38.089479553903345 40.0 38.0 40.0 38.0 40.0 42 38.0846468401487 40.0 38.0 40.0 38.0 40.0 43 38.105947955390334 40.0 38.0 40.0 38.0 40.0 44 38.02193308550186 40.0 38.0 40.0 38.0 40.0 45 38.061412639405205 40.0 38.0 40.0 38.0 40.0 46 37.93092936802974 40.0 38.0 40.0 32.0 40.0 47 37.88256505576208 40.0 38.0 40.0 32.0 40.0 48 37.827026022304835 40.0 38.0 40.0 32.0 40.0 49 37.8475092936803 40.0 38.0 40.0 32.0 40.0 50 37.81862453531598 40.0 38.0 40.0 32.0 40.0 51 37.396133828996284 40.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -1.036008371260941 2201 2 0.45490031983625556 2201 3 1.1062415951614355 2201 4 0.4854956169599305 2201 5 0.9563053764959619 2201 6 0.5978330541461574 2201 7 0.21656693238035984 2201 8 0.49588693594611755 2201 9 0.6120287834293023 2201 10 0.7749529780087983 2201 11 1.0556908671250511 2201 12 1.1065901363794595 2201 13 0.7863116778654771 2201 14 0.6168330540917921 2201 15 0.6376136805614365 2201 16 0.6138847932693707 2201 17 0.3937154472536619 2201 18 0.5520865339149239 2201 19 0.7249196997714407 2201 20 0.2058666200650734 2201 21 0.737320731006271 2201 22 0.9005751400997113 2201 23 0.6869619225578134 2201 24 0.4827813224376669 2201 25 0.4832813215252685 2201 26 0.6126714142266394 2201 27 0.9194313785983681 2201 28 0.6298995194008867 2201 29 0.8240482713383912 2201 30 0.6983108918631231 2201 31 -0.12700045853981834 2201 32 0.4474212419792849 2201 33 0.5381130920333703 2201 34 0.2746797038642015 2201 35 0.6275127872703621 2201 36 0.9938500704375528 2201 37 0.6792876347599162 2201 38 0.45214698370721607 2201 39 0.1156628263999977 2201 40 -0.34046376743981455 2201 41 0.7200549719546459 2201 42 0.6310832370003823 2201 43 0.7606603784894901 2201 44 1.3414769754436193 2201 45 1.0081985198045942 2201 46 0.6782522223780418 2201 47 0.6199727000862794 2201 48 0.5081950848042069 2201 49 0.8208244125777853 2201 50 0.3333183375077695 2201 51 1.2505817903279777 1101 1 0.8739483386957687 1101 2 0.01074447568041137 1101 3 -0.4262259373060999 1101 4 0.8699112013755155 1101 5 0.10955212974270978 1101 6 0.06103651734962057 1101 7 -0.058757742944315794 1101 8 0.20324624330542207 1101 9 0.6873534587539822 1101 10 0.695299298355124 1101 11 0.7301497415839293 1101 12 -0.09773886794954478 1101 13 0.31012120167499546 1101 14 0.6618546991134409 1101 15 -0.024723981776226367 1101 16 0.23726141664599254 1101 17 0.404105057643271 1101 18 -0.1942338124054217 1101 19 0.14136991622165596 1101 20 0.05868047287892608 1101 21 0.1555025491880926 1101 22 -0.2899010503764785 1101 23 0.3337151693110627 1101 24 -0.1726299329735852 1101 25 -0.3132554750115233 1101 26 -0.0462030446478181 1101 27 -0.32126126209427497 1101 28 -0.05235156285019116 1101 29 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24 0.931689003903486 1212 25 0.45086412631737716 1212 26 1.1637637327608203 1212 27 0.7305659804306472 1212 28 0.27190092884416117 1212 29 -0.3063252311281346 1212 30 -0.6392507712799329 1212 31 0.6467838966398816 1212 32 -0.32424894829556195 1212 33 -0.32658035405542307 1212 34 -0.026941155896189173 1212 35 0.43230489438805364 1212 36 0.5350762860823721 1212 37 0.5538471837380712 1212 38 0.050455651783323674 1212 39 -0.06615535541818218 1212 40 0.2599590655411532 1212 41 0.8321057119123623 1212 42 0.4154384167043972 1212 43 0.13980061809484567 1212 44 -0.03836798579668965 1212 45 0.824266173081547 1212 46 0.8981253724837543 1212 47 0.22603330614688844 1212 48 0.8330717585180878 1212 49 0.2147638065171762 1212 50 0.17968902108775353 1212 51 -0.11164513003847532 1213 1 0.3437896085370369 1213 2 -0.4400491751132378 1213 3 -0.4230513341314932 1213 4 0.16832389978821283 1213 5 -0.06187644168586104 1213 6 0.026115882428989323 1213 7 -0.4460593302459017 1213 8 -0.6031029630437814 1213 9 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-0.10296746900083065 1213 45 -0.5271550155489422 1213 46 -0.4207376766118571 1213 47 -0.4184111382975573 1213 48 -0.6170574404483204 1213 49 -0.5407917490383767 1213 50 0.45049005467948433 1213 51 -0.37366063391444726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 1.0 17 0.0 18 0.0 19 0.0 20 2.0 21 6.0 22 8.0 23 16.0 24 31.0 25 55.0 26 99.0 27 120.0 28 177.0 29 249.0 30 305.0 31 367.0 32 460.0 33 563.0 34 701.0 35 925.0 36 1125.0 37 1777.0 38 3551.0 39 16361.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.20977771158357 20.813940499683508 13.687306847376849 27.28897494135607 2 13.360594795539033 23.007434944237918 42.91449814126394 20.71747211895911 3 15.851301115241636 26.32342007434944 35.375464684014865 22.449814126394052 4 11.602230483271375 22.50185873605948 39.44981412639405 26.44609665427509 5 12.118959107806692 36.94423791821561 38.7360594795539 12.200743494423792 6 25.356877323420075 44.286245353159856 18.04460966542751 12.312267657992566 7 22.992565055762082 33.680297397769515 23.509293680297397 19.817843866171003 8 21.260223048327138 41.0631970260223 21.539033457249072 16.13754646840149 9 23.193308550185872 17.684014869888475 21.71747211895911 37.40520446096654 10 13.780669144981413 32.5910780669145 31.605947955390334 22.022304832713754 11 29.557620817843866 25.43122676579926 28.643122676579925 16.36802973977695 12 18.84014869888476 26.788104089219335 31.156133828996285 23.215613382899626 13 23.858736059479554 23.301115241635685 31.438661710037174 21.401486988847584 14 18.84014869888476 22.037174721189594 28.263940520446095 30.858736059479554 15 20.765799256505577 28.977695167286242 24.635687732342006 25.620817843866174 16 23.970260223048324 29.87360594795539 25.765799256505577 20.390334572490705 17 20.286245353159853 32.77695167286245 27.37918215613383 19.557620817843866 18 24.33085501858736 28.821561338289964 27.315985130111525 19.531598513011154 19 21.349442379182157 27.79553903345725 27.30111524163569 23.55390334572491 20 20.115241635687735 27.49442379182156 27.289962825278813 25.1003717472119 21 20.144981412639403 27.122676579925653 27.650557620817846 25.0817843866171 22 19.992565055762082 31.349442379182157 27.96282527881041 20.695167286245354 23 20.271375464684017 27.93680297397769 27.53531598513011 24.25650557620818 24 24.018587360594797 28.345724907063197 27.36802973977695 20.267657992565056 25 20.438661710037177 31.412639405204462 28.182156133828997 19.966542750929367 26 24.092936802973977 27.873605947955387 27.858736059479554 20.17472118959108 27 20.375464684014872 32.49442379182156 27.401486988847584 19.728624535315987 28 19.28624535315985 28.550185873605948 27.44609665427509 24.71747211895911 29 22.063197026022305 27.776951672862456 29.07806691449814 21.0817843866171 30 19.970260223048328 30.48698884758364 29.475836431226764 20.066914498141266 31 21.353159851301115 27.029739776951672 28.17472118959108 23.442379182156134 32 22.78810408921933 27.07434944237918 27.773234200743495 22.364312267657994 33 19.799256505576206 28.126394052044613 29.25650557620818 22.817843866171003 34 21.04832713754647 27.133828996282528 30.869888475836433 20.94795539033457 35 21.260223048327138 30.04089219330855 28.1635687732342 20.53531598513011 36 20.059479553903344 29.613382899628256 29.962825278810406 20.36431226765799 37 19.776951672862452 31.90706319702602 27.754646840148702 20.561338289962823 38 19.304832713754646 27.16728624535316 32.52416356877323 21.00371747211896 39 19.605947955390334 27.204460966542747 28.855018587360593 24.334572490706318 40 19.66914498141264 26.171003717472118 32.70631970260223 21.453531598513013 41 19.62081784386617 26.53159851301115 29.03717472118959 24.810408921933085 42 23.568773234200744 25.977695167286246 28.576208178438662 21.877323420074347 43 20.070631970260223 26.594795539033456 32.66171003717472 20.6728624535316 44 19.12639405204461 30.208178438661708 29.096654275092938 21.568773234200744 45 19.29368029739777 26.643122676579928 32.8364312267658 21.226765799256505 46 22.79553903345725 26.163568773234203 29.762081784386616 21.278810408921935 47 18.739776951672862 26.643122676579928 28.992565055762082 25.62453531598513 48 18.54275092936803 26.312267657992567 28.69888475836431 26.44609665427509 49 22.828996282527882 25.96282527881041 29.02230483271375 22.185873605947958 50 18.8364312267658 26.133828996282528 32.6542750929368 22.37546468401487 51 17.72118959107807 26.565055762081784 29.312267657992564 26.401486988847584 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 3.0 6 6.0 7 3.5 8 1.0 9 2.5 10 4.0 11 7.0 12 10.0 13 22.5 14 35.0 15 56.5 16 78.0 17 85.0 18 92.0 19 82.5 20 73.0 21 68.5 22 64.0 23 89.0 24 114.0 25 139.0 26 247.0 27 330.0 28 501.5 29 673.0 30 839.0 31 1005.0 32 1304.5 33 1604.0 34 1879.0 35 2154.0 36 2379.0 37 2604.0 38 2635.0 39 2666.0 40 2509.0 41 2352.0 42 2235.0 43 2118.0 44 1945.0 45 1772.0 46 1633.0 47 1494.0 48 1409.0 49 1324.0 50 1336.5 51 1349.0 52 1657.0 53 1965.0 54 1363.0 55 761.0 56 630.5 57 500.0 58 440.5 59 381.0 60 321.5 61 262.0 62 224.0 63 186.0 64 169.5 65 153.0 66 167.5 67 182.0 68 159.0 69 136.0 70 116.5 71 97.0 72 98.0 73 99.0 74 76.5 75 33.0 76 12.0 77 13.5 78 15.0 79 12.0 80 9.0 81 5.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15985130111524165 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 26900.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.242777722435 #Duplication Level Percentage of deduplicated Percentage of total 1 96.59895375500325 87.17357911932537 2 2.339988050816126 4.223340430859071 3 0.5354675226336139 1.4496622986782444 4 0.17838803466670544 0.6439292704307811 5 0.13515134640171117 0.6098216456108718 6 0.05493325289335416 0.2974397598255161 7 0.03095837130734487 0.19556385943761326 8 0.025938113530236236 0.18725819310787137 9 0.02554696350187754 0.20748860539047848 >10 0.054045877883236594 0.8196111523570674 >50 0.012377226821086752 0.6813427505286651 >100 0.004125742270729122 1.2919449960907947 >500 0.004125742270729122 2.219017918357676 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 596 2.2156133828996283 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 347 1.2899628252788105 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT 76 0.2825278810408922 No Hit TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT 54 0.2007434944237918 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 53 0.19702602230483268 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 42 0.15613382899628253 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.11895910780669146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0037174721189591076 2 0.0 0.0 0.0037174721189591076 3 0.0 0.0 0.0037174721189591076 4 0.0 0.0 0.0037174721189591076 5 0.0 0.0 0.0037174721189591076 6 0.0 0.0 0.0037174721189591076 7 0.0 0.0 0.0037174721189591076 8 0.0 0.0 0.0037174721189591076 9 0.0 0.0 0.0037174721189591076 10 0.0 0.0 0.0037174721189591076 11 0.0 0.0 0.007434944237918215 12 0.0 0.0 0.007434944237918215 13 0.0 0.0 0.007434944237918215 14 0.0 0.0 0.007434944237918215 15 0.0 0.0 0.011152416356877323 16 0.0 0.0 0.011152416356877323 17 0.0 0.0 0.011152416356877323 18 0.0 0.0 0.011152416356877323 19 0.0 0.0 0.011152416356877323 20 0.0 0.0 0.011152416356877323 21 0.0 0.0 0.01486988847583643 22 0.0 0.0 0.022304832713754646 23 0.0 0.0 0.03345724907063197 24 0.0 0.0 0.055762081784386616 25 0.0 0.0 0.055762081784386616 26 0.0 0.0 0.05947955390334572 27 0.0 0.0 0.06691449814126393 28 0.0 0.0 0.11524163568773234 29 0.0 0.0 0.20817843866171004 30 0.0 0.0 0.37174721189591076 31 0.0 0.0 0.6802973977695167 32 0.0 0.0 0.9479553903345725 33 0.0 0.0 1.211895910780669 34 0.0 0.0 1.513011152416357 35 0.0 0.0 1.8736059479553904 36 0.0 0.0 2.2602230483271377 37 0.0 0.0 2.9144981412639406 38 0.0 0.0 3.684014869888476 39 0.0 0.0 4.516728624535316 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 135 0.0 40.0 45 ATGCCGT 150 0.0 36.0 44 TGCTGGA 70 1.8189894E-11 35.357143 30 GTATGCC 160 0.0 33.75 42 TATGCCG 160 0.0 33.75 43 CGTATGC 160 0.0 33.75 41 TCGTATG 160 0.0 33.75 40 CTCGTAT 160 0.0 33.75 39 CGAGACT 95 0.0 33.157894 23 GCTGGAA 75 4.1836756E-11 33.0 31 CTGGAAT 75 4.1836756E-11 33.0 32 CTTGCTG 75 4.1836756E-11 33.0 28 GACTTGC 75 4.1836756E-11 33.0 26 TTGCTGG 75 4.1836756E-11 33.0 29 AGCCCAC 165 0.0 32.727272 17 ATCTCGT 165 0.0 32.727272 37 GCCCACG 165 0.0 32.727272 18 CCACGAG 165 0.0 32.727272 20 CACGAGA 165 0.0 32.727272 21 CCCACGA 165 0.0 32.727272 19 >>END_MODULE