FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_39.34100000009b30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_39.34100000009b30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26900
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC5962.2156133828996283No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC3471.2899628252788105No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT760.2825278810408922No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT540.2007434944237918No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC530.19702602230483268No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC420.15613382899628253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.11895910780669146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1350.040.045
ATGCCGT1500.036.044
TGCTGGA701.8189894E-1135.35714330
GTATGCC1600.033.7542
TATGCCG1600.033.7543
CGTATGC1600.033.7541
TCGTATG1600.033.7540
CTCGTAT1600.033.7539
CGAGACT950.033.15789423
GCTGGAA754.1836756E-1133.031
CTGGAAT754.1836756E-1133.032
CTTGCTG754.1836756E-1133.028
GACTTGC754.1836756E-1133.026
TTGCTGG754.1836756E-1133.029
AGCCCAC1650.032.72727217
ATCTCGT1650.032.72727237
GCCCACG1650.032.72727218
CCACGAG1650.032.72727220
CACGAGA1650.032.72727221
CCCACGA1650.032.72727219