FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_38.34100000009b23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_38.34100000009b23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46920
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC6671.4215686274509804No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC4020.8567774936061381No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT1080.23017902813299232No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT670.14279624893435636No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT630.13427109974424553No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA560.11935208866155159No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTG206.964225E-445.044
TGCCGTC1600.040.7812545
CTATATC900.040.00000433
GCTATAT900.040.00000432
CGCTATA900.040.00000431
GACGCTA900.040.00000429
GACGACG900.040.00000426
AGACGAC950.037.89473725
ACGCTAT950.037.89473730
CGAGACG950.037.89473723
ATATCTC1050.036.4285735
TATATCT1000.036.034
GAGACGA1000.036.024
ACGACGC1000.036.027
ATGCCGT1900.035.52631444
TCTCCGA2000.034.87511
TATCTCG1100.034.77272836
CTCCGAG1950.034.61538312
CGACGCT1050.034.28571328
CTCGTAT2000.033.7539