FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_37.34100000009b09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_37.34100000009b09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601908
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG10330.17162091216597886No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC8690.14437422330322905No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC7000.11629684270685885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG1350.034.99902718
CCCACGA1600.029.53043219
TATGCCG1700.029.1168443
ACTGTGC554.161502E-628.635578
TAAGACT952.3646862E-1128.4202654
TCGTATG1750.028.28493140
TACCCGG2300.027.3905456
ATGCCGT1900.027.23608844
ACCTATA2400.027.2206651
CGCTATA1004.5474735E-1126.99925431
CGTCGGG2550.026.46985614
CTCCGAG2050.026.34073412
ATACCCG2650.026.3200245
CGTATGC1900.026.0519141
CTCGTAT1900.026.0519139
CGAGACG1650.025.90837523
TGCCTAC1400.025.7456551
GCCTCGA2600.025.09545932
GACGCTA1101.5097612E-1024.54477529
CGGGGCA3250.024.23009917
Produced by FastQC (version 0.11.2)