FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_36.34100000009afb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_36.34100000009afb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529970
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC11400.2151065154631394No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG7330.13830971564428174No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC5540.10453421891805197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1300.034.61422343
GCCCACG1400.033.74886718
CGTATGC1400.033.74886741
GTATGCC1450.031.03344342
ATGCCGT1450.031.03344344
CCCACGA1600.029.53025619
GAGACCC1700.027.79318624
CGAGACC1700.026.469723
GGATAAT1800.026.24911932
CTCGTAT1850.025.53968239
TCGTATG1850.025.53968240
TGGCCCC450.001225922324.999169
AATCTCG1800.024.9991636
TCTCGTA1900.024.86758638
CGGCAGG1101.5097612E-1024.5446335
CTACGCT551.3671558E-424.544634
ACGTTAG651.5044236E-524.2665881
GATAATC2000.023.62420833
CACGAGA2100.023.57063921
AGACCCT2100.023.57063925
Produced by FastQC (version 0.11.2)