FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_35.34100000009aee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_35.34100000009aee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260031
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC59500.47221060434227413No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT17980.14269490195082501No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA13500.10714022115328907No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7900.039.5871445
TATGCCG9600.032.34255243
ATGCCGT9600.032.34255244
GTATGCC9900.031.5897442
GCCCACG10200.030.4400518
CGTATGC10300.030.36295141
CCCACGA10300.030.14451219
CCACGAG10350.029.9988920
CTCGTAT10750.029.09194439
CGAGACA10300.029.0523223
ACGAGAC10950.028.76605822
TCGTATG11200.028.5257340
TATCTCG10900.028.48518236
CACGAGA11000.028.43076521
TCTCGTA11200.028.12395938
AGCCCAC11300.027.4768617
CGAGCCC11750.027.19048315
TACCCGG507.160743E-526.9996
CCGAGCC11800.026.88459614
GAGCCCA11950.026.17057816
Produced by FastQC (version 0.11.2)