FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_34.34100000009ad1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_34.34100000009ad1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038765
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC39860.383724904092841No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA12720.12245310537031956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGC451.9284926E-839.9986766
TGCCGTC6000.036.3737945
ACGGGCT250.002107358335.99880616
TATGCCG6100.035.777543
ATGCCGT6250.035.27882844
CGAGACT6100.034.6709823
CCACGAG6300.034.6417120
ACGAGAC6450.034.18491422
CGTATGC6600.033.06708541
CACGAGA6650.032.81846221
TCGTATG6650.032.81846240
GTATGCC6950.032.04929742
ATCTCGT6850.031.86025837
CCCACGA6900.031.62938919
GACTTGC6550.031.60200526
CTCGTAT6950.031.40183839
TCTCGTA7250.030.41278538
GCCCACG7200.030.31149718
GCGCGTT300.00514786829.9990069
TGACCCG300.00514786829.99900612
Produced by FastQC (version 0.11.2)