FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_33.34100000009ac4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_33.34100000009ac4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424883
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC66200.46459954957705296No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT19530.13706388524531488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8450.041.0047345
TGCGCGC301.14025286E-437.49891710
ATGCCGT10250.033.803944
ACGACGC10150.032.80693427
GCGCGCT551.1283191E-732.7263311
CGACGCT10300.032.32916328
CGCTATA10400.032.23464631
CTGCGCG352.8211923E-432.141939
GCCCACG10650.032.11174818
CCCACGA10750.032.0223319
TATGCCG10900.031.78807443
GTATGCC10850.031.72719442
CGTCCCG501.9825056E-631.49909225
CGCCACG501.9825056E-631.49909220
CGAGACG10700.031.3308723
CGAGCCC10950.031.23197615
GACGCTA10700.031.12059629
AGCCCAC11250.030.59911517
CCGAGCC11200.030.53483214
GTCCCGG602.4202745E-729.99913426
Produced by FastQC (version 0.11.2)