FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_32.34100000009ab7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_32.34100000009ab7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384804
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC45110.32575007004601375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5600.036.96250545
GCCCACG5850.035.3829118
CCCACGA6250.033.4783919
CCGAGCC6350.032.59685514
AGCCCAC6400.032.34219417
CGAGCCC6400.032.34219415
TATGCCG6350.032.24254243
ATGCCGT6450.031.74265944
CAAGGCG6400.031.63910329
GGCGAAT6450.031.39383932
CGAGACC6500.031.15234823
GTATGCC6800.030.10884542
CGTATGC6900.029.99855641
GGCACGC300.00514883129.99855622
GCGAATC6700.029.8866233
AGGCGAA6950.029.45901331
GGGTGCC554.1673084E-628.6349859
CCACGAG7350.028.46801620
GAGCCCA7400.028.27566516
TCCGAGC7400.027.97162613
Produced by FastQC (version 0.11.2)