FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_31.34100000009aab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_31.34100000009aab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458384
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC92500.6342636781533533No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT15360.10532205509660009No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10450.041.76858545
GCCCACG12450.035.420218
CCGAGCC12550.035.31724514
TATGCCG12650.034.860243
CGAGCCC12700.034.72295415
ATGCCGT12850.034.31762744
CCCACGA13050.033.79168719
AGCCCAC13100.033.66271217
TAACCTA13000.033.0563131
CGAGACG13350.032.86379223
TCCGAGC13550.032.71080813
GTATGCC13550.032.5447642
GAGCCCA13650.032.3063416
CCACGAG13650.032.3063420
CGTATGC13750.032.07138441
ACGAGAC14450.030.82915922
TCGTATG14300.030.68053240
CTCCGAG14550.030.61727312
TATCTCG14150.030.52875536
CTCGTAT14300.030.3658639
Produced by FastQC (version 0.11.2)