FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_29.34100000009a81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_29.34100000009a81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444219
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC38190.2644335796717811No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4400.038.3511545
CGCGCGC250.0021075935.99894310
ATGCCGT5550.030.40451444
GCGCGCT453.4921548E-529.99912311
TCGCGCG300.005148427629.9991239
GCCCACG5650.029.86638518
CGACCTA5800.029.48189531
TATGCCG5750.029.34696643
GCGACCT5700.029.20967530
CTGCGAC5750.028.5643828
GACTGCG5850.028.07610326
CCGAGCC6200.027.21694614
TGCGACC6100.026.92544429
CGAGCCC6350.026.57402615
GACCTAT6400.026.36641532
CCCACGA6450.026.16202519
AGCCCAC6600.025.56743417
ACTGCGA6550.025.41910427
GTATGCC6650.025.37519842
CCACGAG6700.025.1858320
Produced by FastQC (version 0.11.2)