FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_28.34100000009a74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_28.34100000009a74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493267
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC459773.0789537303107886No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT99620.6671278478664565No Hit
GTTCCAAAGTGTTCACTTCTTTTTCGCCAGGTTTCATTCGATTTATTGTGG29990.20083481386784816No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG25970.1739139751966661No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG23060.15442650242722836No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG21810.14605559488021902No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGG20220.13540780048042314No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA19330.12944771430695248No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA19330.12944771430695248No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA17180.11504975332609642No Hit
CTCTAAGCCTAAGTATTCCTCAATGACCGATAGCGTACAAGTACCTGTCTC17140.1147818842845921No Hit
GTACTTGTACGCTATCGGTCATTGAGGAATACTTAGGCTTAGAGCTGTCTC16140.10808515824698463No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA15320.1025938428961465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC51550.043.90738745
ATGCCGT62150.036.49116544
AGCCGGT250.002107648635.998826
CCACGAG64350.035.2086420
CTTGCAG63950.035.18258729
CCCACGA64700.035.08772319
TATGCCG64700.035.05294843
GTATGCC64750.035.0258842
CCGACTA458.7044646E-734.9988569
CCTTGCA64350.034.99885628
CGTATGC65050.034.9681141
GCCCACG65050.034.86434618
TCGTATG65550.034.77002740
GACCTTG65100.034.73388726
ACCTTGC65250.034.65403727
ACGAGAC65850.034.57745722
TGCAGAA65050.034.48388331
CTCGTAT66100.034.4126439
TTGCAGA65400.034.3681430
AGACCTT66000.034.2943325
Produced by FastQC (version 0.11.2)