FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_27.34100000009a67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_27.34100000009a67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316692
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC55940.424852585114818No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA14140.10739033881879742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7000.037.9266845
ATGCCGT7700.034.478844
ACTGCGC401.5612712E-533.7483188
TATGCCG7950.033.6775743
GCCCACG8100.033.0539118
CGTATGC8350.032.0642741
CCCACGA8350.032.0642719
CGAGACT8350.032.0642723
CCACGAG8350.032.0642720
GTATGCC8850.030.25273142
AGCCCAC8900.030.08277117
CACGAGA8900.030.08277121
GCGCGCT602.4204928E-729.99850711
CCGAGCC9200.029.5909214
TCGTATG9100.029.42161240
AGACTAG9250.029.18773725
ACGAGAC9250.029.18773722
CGAGCCC9300.028.78888915
CTCGTAT9400.028.24327539
GGTCGCG406.168422E-428.12367
Produced by FastQC (version 0.11.2)