FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_24.34100000009a24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_24.34100000009a24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302621
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC8380.2769140277773188No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.18108459095700563No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3420.11301264618119695No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC3300.10904729017483916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.10673416583779712No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3090.10210791716371304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCAAT250.002086361636.0640951
ATGCCGT1400.033.7486444
TATGCCG1400.032.1415643
TCTCGTA1450.031.0332338
GGTGCGA453.4833993E-529.99878920
TGTACCC300.00514235329.9987895
CTCGTAT1500.029.99878739
GTATGCC1500.029.99878742
CGTATGC1550.029.03108441
CCCACGA1600.028.12386319
CGGATGG803.3760443E-928.12386334
CGTCGGG1200.028.12386314
GGATAAT1450.027.92990732
GCCCACG1700.027.79299518
ACCTATA1300.027.7416111
CGGCCGT1350.026.6655910
CCTGGAT1650.025.90804729
TCGTATG1850.025.53950940
TATTAAA450.001224755324.9989912
CTTTAGG450.001224755324.9989912
Produced by FastQC (version 0.11.2)