FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_23.34100000009a17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_23.34100000009a17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575244
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC27510.47823184596449503No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG9300.1616705258985752No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA8020.13941909867812616No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC7670.1333347240475346No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT6770.11768918928315637No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC5940.10326052944489644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG300.005145826429.9991879
ATGCCGT3500.029.57063144
CGTATGC3550.028.52035541
TATGCCG3700.027.36412443
GTATGCC3800.026.64401842
CGAGACA3550.026.61923
GCCCACG3850.026.2979918
CCCACGA3900.025.96083619
CTCGTAT4000.025.874339
TCGTATG4050.025.55486540
ACGAGAC4150.025.48123722
TATCTCG3900.025.38392836
TCTCGTA4400.024.0334438
TAACCGG1305.0931703E-1122.49939344
TAGGACC500.002263600522.4993924
TACTGTA602.465882E-422.499392
ATCTCGT4850.021.80353437
CCGAGCC4750.021.78888514
CTCCGAG4800.021.56191612
CACGAGA4700.021.54197121
Produced by FastQC (version 0.11.2)