FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_22.34100000009a0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_22.34100000009a0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048796
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTATAAGAGTGCTAATGTAAGTGACAGATATCTTTTTCACGCCATCATA34860.332381130362816No Hit
AATTATGATGGCGTGAAAAAGATATCTGTCACTTACATTAGCACTCTTATA30820.2938607698732642No Hit
ATCCAAAGTTGCATATTCAATCTCTGACTTAACAGAATCAATCTCTGCTTG26810.25562645166457537No Hit
ACCTTAGGTGAAAGGTTAAAGAATCTGAATTGAACCTCAAGATGTGCAAAA26590.25352880827158003No Hit
TCATCATTCATACCATCAAATCCTAAAGCACACAACAACTGAAAAAACAAA22250.21214802497339807No Hit
CATTCATACCATCAAATCCTAAAGCACACAACAACTGAAAAAACAAACACC19930.19002742191999208No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC19800.18878790536958567No Hit
GTGATAGACTATCCAAATGAGTATTTGATATCAGTGGAGGGAACCTACAAC19100.18211358548278217No Hit
CTTCTCTACTCTCCAATGACTGTAAAACTTGCATTTTCTCTGAGTCACAAG17870.1703858519673988No Hit
GATCAAAACAAACCAACTTTCCTTCTCTAATCAAAACAAGAAAGACACAAA14790.14101884446546326No Hit
GTAGATATGAGAGCCTTAAAACAAGATGAAGTTCGTGACTATATTTCCATC14690.14006537019591989No Hit
ATCCATAGCTACAAAGTACCATCAGATGATTGTTTTGCACATCTTGAGGTT14320.13653751539860945No Hit
ATCATGATCAATGTGGTTCCAGTGACAAAAGAAGACGACAGAATCCATAGC13870.13224688118566433No Hit
ATAGAAAGGTGTTTGTTTTTTCAGTTGTTGTGTGCTTTAGGATTTGATGGT13240.12623999328754115No Hit
ATCCTAAAGCACACAACAACTGAAAAAACAAACACCTTTCTATCTTCTTTG12830.12233074878241336No Hit
CTGTAAAACTTGCATTTTCTCTGAGTCACAAGCAGAGATTGATTCTGTTAA11930.1137494803565231No Hit
TCATTCATACCATCAAATCCTAAAGCACACAACAACTGAAAAAACAAACAC11730.11184253181743638No Hit
CTCCAATGACTGTAAAACTTGCATTTTCTCTGAGTCACAAGCAGAGATTGA11630.11088905754789301No Hit
GTTTTATAGCCTTTATAGTCTCGAATTACGAAGGAATTGGAGAGAAGTAAA11230.10707516046971957No Hit
AGGTTAAAGAATCTGAATTGAACCTCAAGATGTGCAAAACAATCATCTGAT11040.10526355935758717No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCT250.002107397235.99870311
TTAGGTA3300.034.7714734
GTCTTAG3500.033.481641
TATGCCG3400.032.42530443
AAGCGCC352.8208058E-432.141719
TAGGTAA3600.031.8738525
GCCCACG3550.031.68918
CCCACGA3550.031.68919
TGCCGTC3550.031.0552245
CGAGCCC3650.030.82080715
TTGCTGG3900.030.5758229
CGTATGC3700.030.40431241
AGCCCAC3700.030.40431217
CCACGAG3700.030.40431220
GACTTGC3750.029.99891926
GTCAGGA300.00514796229.9989199
CCGAGCC3750.029.99891914
GAGCCCA3750.029.99891916
ATGCCGT3700.029.79622744
TCCGAGC3850.029.21972713
Produced by FastQC (version 0.11.2)