FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_21.341000000099fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_21.341000000099fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832530
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC28010.3364443323363722No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA11400.13693200245036213No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT9370.11254849675086784No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA9270.1113473388346366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG3800.036.1169318
ATGCCGT3900.035.76775444
CGACGCT3700.035.26881428
CCCACGA3900.035.19085319
CGCTATA3750.034.7985631
TATGCCG3950.034.74539643
CGTATGC4050.033.88748641
GTATGCC4100.033.47422442
GACGCTA3900.033.46015529
ACGACGC3900.033.46015527
CGAGACG4050.032.7764223
TGCCGTC4350.032.0676445
CCACGAG4350.031.55041720
AGACGAC4150.031.4444825
GACGACG4250.030.70461326
ACGCTAT4300.030.34758230
GCGGCAC300.005147493429.99875819
CACGAGA4650.029.51490821
CTCGTAT4700.029.20092239
AGCCCAC4800.028.59256617
Produced by FastQC (version 0.11.2)