FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_20.341000000099ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_20.341000000099ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682919
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC15290.2238918524744516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2250.031.99880218
CAAGGCG2250.031.99880229
TATGCCG2400.030.93634443
CCCACGA2350.030.63715219
AGGCGAA2600.030.28732731
GTATGCC2600.029.42197442
TGCCGTC2500.028.79892345
AAGGCGA2600.028.55662230
GGCGAAT2550.028.23423832
ACGAGAC2550.028.23423822
CGAGACC2750.027.8171423
ATGCCGT2650.027.16879544
CGTATGC2750.026.9989941
GCGAATC2700.026.6656733
CCGAGCC2700.026.6656714
CTCGTAT2850.026.05165739
TCGTATG2900.025.60248840
CCACGAG2850.025.26221320
CACGAGA2950.024.40586721
GTATACG500.002242099322.537111
Produced by FastQC (version 0.11.2)