FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_2.3410000000980c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_2.3410000000980c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314094
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.211083306271371No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5400.17192305488165963No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC5080.1617350219997835TruSeq Adapter, Index 12 (95% over 21bp)
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4980.15855126172419723No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.15250211720058326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.1397670760982381No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4030.12830553910612746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.10952135348016837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG556.002665E-1140.90764643
GCCCACG702.0008883E-1135.35589218
CCCACGA653.6561687E-1034.6141619
ATGCCGT653.6561687E-1034.6141644
TCGTATG708.094503E-1032.1417240
TCTAGAC300.005142520629.998942
GTATGCC751.6934791E-929.99893842
CTCGTAT852.0190782E-1029.11661739
GTTAGGC702.8730938E-828.9275473
TCTCGTA903.929017E-1027.4990338
CGTATGC856.4464984E-926.46965241
GTCTAGA608.068553E-626.2909281
CGGCAGG608.169043E-626.24907135
TTCGGCA608.169043E-626.24907133
AGGGCAG608.169043E-626.2490715
TCGGCAG651.5195905E-524.2299134
TACCCGG851.8851824E-723.8226876
CAGGCCT851.8851824E-723.82268729
GACTACT851.8851824E-723.82268726
ATCTCGT952.1038431E-823.6833737
Produced by FastQC (version 0.11.2)