FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_19.341000000099d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_19.341000000099d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872260
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC34860.39965148006328394No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA9740.11166395340838742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4250.039.7047745
CCGGCCA250.002107120435.998999
ATGCCGT4900.034.4378144
GCCCACG5050.033.8604418
TATGCCG5000.033.74905443
CGAGACG5000.033.74905423
CCCACGA5150.033.20295319
GTATGCC5100.033.08730742
CGTATGC5200.032.4510141
CCACGAG5350.031.9617220
GCGGTAC855.456968E-1231.76381542
TATCTCG5450.031.37526736
ACGAGAC5550.031.21534322
CCGAGCC5750.030.12959114
CGGGTGG300.005147290429.999164
AGCCAGG602.4184374E-729.9991626
CGCGGTA901.2732926E-1129.99915941
AGCCCAC5800.029.48193417
CACGAGA6050.029.00745221
TAACCTA5950.028.7386931
Produced by FastQC (version 0.11.2)