FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_18.341000000099c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_18.341000000099c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796774
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC28840.3619596020954499RNA PCR Primer, Index 46 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG3600.036.24878718
TGCCGTC3600.036.24878745
CGAGACC3700.035.2690923
CCCACGA3800.034.9330419
TATGCCG3800.034.34095843
ATGCCGT3900.033.4604244
GTATGCC3950.033.0368742
CGTATGC4000.032.62390541
CCACGAG4000.032.62390520
AGCCCAC4050.032.2211417
CCGAGCC4050.032.2211414
TCGTATG4150.031.4447340
GTACGAC300.00514716529.9989973
CGGACGT300.00514716529.99899720
ACGAGAC4450.029.83046222
TCCGAAT4300.029.82458532
CGAGCCC4400.029.65809615
CTCGTAT4450.029.32486239
GAGCCCA4550.029.1748516
CATTCCG4500.028.49904429
Produced by FastQC (version 0.11.2)