FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_17.341000000099b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_17.341000000099b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866411
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC19220.22183467199747No Hit
GACTAGGATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGA16690.19263375003318287No Hit
GTTGTAAGATGGGATAACGCAGTATCATCTGTGTTATCTCTGTCCTGTGTT13320.1537376603020968No Hit
ATATTTCATTGACTAGGATAGATAGGAGAGTTGTAACACAGGACAGAGATA9510.10976314935983039No Hit
AGGATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGATACT9190.10606975211533556No Hit
GGATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGATACTG8970.10353054150974539No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCT207.0333027E-444.9987315
TGCGACC2750.031.0900329
CACGGAC300.005147278329.99915322
CGACCTA2850.029.99915331
TGCCGTC2850.029.99915345
TATGCCG2900.029.48192643
GACTGCG2900.029.48192626
CGAGACT2950.028.98223123
CGAGCCC3200.028.12420515
ATGCCGT3050.028.03199644
CCACGAG3150.027.85635620
GCGACCT3100.027.57986630
CCCACGA3200.027.421119
ACGAGAC3250.026.99923722
CTGCGAC3250.026.30694828
TATCTCG3250.026.30694836
TCTCGTA3250.026.30694838
CCCTTAC1200.026.2826271
CGTATGC3300.025.9083641
TCCGAGC3400.025.80809413
Produced by FastQC (version 0.11.2)