FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_16.341000000099ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_16.341000000099ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678760
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC231413.4093052036065767No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT82981.2225234250692438No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA37430.551446755848901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA26420.3892392008957511No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC23510.3463669043550003No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC23180.34150509753079145No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC19950.29391832164535325No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC19080.28110083092698446No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC18930.27889091873416233No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA18700.27550238670516825No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG18670.27506040426660383No Hit
AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA16640.24515292592374327No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC15940.23484000235723967No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA15610.2299781955330308No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA14270.21023631327715245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG14270.21023631327715245No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG12030.17723495786434085No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGA11920.17561435558960456No Hit
AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG11100.16353350226884317No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG10540.1552831634156403No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10520.15498850845659734No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAACTGTCTC10300.15174730390712476No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG10170.14983204667334551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTCTGTCTCTTATACACATCTCCG9810.1445282574105722No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC9810.1445282574105722No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAACTGTCT9310.1371618834344982No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC9290.13686722847545524No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA9040.13318404148741822No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG8960.1320054216512464No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAACTGTC8660.12758559726560198No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG8360.12316577287995757No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA8010.11800931109670575No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAACTGTCT7890.11624138134244799No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7880.11609405386292651No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTAT7830.11535741646531911No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAACTGTCTCTTA7770.11447345158819022No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA7720.11373681419058283No Hit
AAAAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG7700.11344215923153986No Hit
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC7690.11329483175201839No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA7620.11226353939536803No Hit
GTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAG7500.11049560964111027No Hit
GTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCC7360.10843302492780954No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTAT7070.10416052802168661No Hit
GTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGCC6910.10180328834934291No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC253.889826E-544.99860435
TCTCGGT207.032321E-444.998613
TGCCGTC24750.042.2714145
ATAGACG250.002106823535.9988828
CTAGGGG1000.033.748954
CACGTCA352.8196836E-432.14185713
GACTACC501.9805193E-631.4990227
ATGCCGT33500.031.43185844
GTATGCC34400.030.2824942
TATGCCG34450.030.23853943
CACGAGA34750.030.23647121
CCACGAG34800.030.19302720
CGAGACC34800.030.12837423
CGTATGC34600.030.04241841
CTTGCAG35000.030.02049429
CCTAGTG300.00514657329.9990713
TACGGTA300.00514657329.999072
CCTCGGG300.00514657329.9990731
TTACGGT300.00514657329.9990712
ACGAGAC35100.029.93496722
Produced by FastQC (version 0.11.2)