FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_15.3410000000999f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_15.3410000000999f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436215
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC24460.5607326662311016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.27348899052072945No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8380.19210710314867668No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA8040.18431278154121247No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7770.17812317320587326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.17674770468690898No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7260.16643169079467693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.1524477608518735No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5640.1292940407826416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3700.037.09317844
TATGCCG3650.036.9848943
CCCACGA3650.036.9848919
AGACTAG3500.036.64145725
TCCTATC3550.035.491633
GCCCACG3750.035.39864718
GTATGCC3850.035.06359542
TCTCGTA3850.035.06359538
GACTAGA3700.034.6608426
CGAGACT3800.034.34079423
ATCTCGT3950.034.1759137
CGTATGC3950.034.1759141
CTCGTAT3950.034.1759139
GAGACTA3800.033.7487124
ACGAGAC4050.033.3320622
TATCTCG3950.033.03671336
CTAGATC4000.032.62375628
CACGAGA4150.032.52887721
CCTATCT4050.032.22099334
TCGTATG4300.031.91738740