FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_14.34100000009982.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_14.34100000009982.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362553
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC7660.21127945431426579TruSeq Adapter, Index 12 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.1199824577372136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG1400.036.9630418
TATGCCG1400.036.9630443
ATGCCGT1550.034.83753244
CGTATGC1550.033.3859741
GTATGCC1600.032.3426642
TCGTATG1600.032.3426640
CGAGACT1550.031.93440623
CTCGTAT1650.031.36257739
CCCACGA1650.031.36257719
ACTACTT1600.030.93645727
GACTACT1650.029.99898726
GAGACTA1700.029.11666524
TACTTGC1750.026.99909229
CTACTTG1850.026.75585428
ACGAGAC2000.025.87412522
AATCTCG1850.025.53967936
TTGCAAT1850.025.53967932
CTAGACC450.001225175924.9991573
GCAATCT1950.024.22995234
ATCTCGT2200.023.52193637
Produced by FastQC (version 0.11.2)