FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_13.34100000009968.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_13.34100000009968.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719394
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC7400.10286435527680242RNA PCR Primer, Index 27 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG1050.034.28412618
TATGCCG1050.032.14136543
CGAGACG1400.025.71309323
ATGCCGT1400.025.71309344
ACGAATT1500.025.49881727
CCCACGA1450.024.82643519
CGTATGC1400.024.10602441
GGTATCA5000.023.447811
GTATGCC1450.023.27478242
CCTAGAC602.4670386E-422.4989553
GACGAAT1750.021.85612926
GTATCAA10950.021.4135271
TCGTATG1601.8189894E-1221.09277240
TCGCGCG1301.2259989E-920.7682699
CTCGTAT1653.6379788E-1220.45359839
GCGCGAC550.00393618420.45359811
TTAGACG550.00393618420.4535984
GTGCTAG908.100025E-620.0408631
CGCGCGC1351.9608706E-919.99907310
ACGGGAT1351.9608706E-919.99907316
Produced by FastQC (version 0.11.2)