FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_11.34100000009925.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_11.34100000009925.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447495
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG250.00210617335.9984789
GCCTTAG406.0935726E-428.1773411
CTATAGT450.001225685824.9989434
TTAGGCG702.69911E-522.499054
ACAGGAC500.002263020522.499053
CCGTTAG654.1798636E-420.8078841
GGTATAT550.003890704820.492611
AACGCAG5250.020.1420086
GTATCAA5700.019.7735711
CTGTAAG706.8876054E-419.3216041
ACGCAGA5750.018.7818157
AGGCGCG600.006506012318.7492086
TAGGCGC600.006506012318.7492085
GGCGCGT600.006506012318.7492087
GGTATCA2050.018.693261
GTGTAAT851.18533324E-418.5638941
TCAACGC5850.018.4607584
CAACGCA5800.018.2319895
CGCAGAG5800.017.8440748
TATCAAC6250.017.6392562
Produced by FastQC (version 0.11.2)