FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_10.3410000000990b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_10.3410000000990b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357149
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG4660.13047775578260054No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC4000.11199807363313351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTATA300.00514350229.9988822
CCCACGA507.146639E-526.99899119
TCGCCCC608.173267E-626.24902225
GTATATA957.239578E-1026.0955051
CGATTAG801.0499207E-725.31155614
CGTATGC450.001225165824.99906741
TACACAG1101.5097612E-1024.5445385
CTCGTAG651.5006914E-524.2706431
ATTAGTC651.5203725E-524.22986416
TACCCGG851.8867286E-723.822646
CGAGTGT802.9661987E-622.4991614
TAGACAG802.9661987E-622.499165
TCCCGCA903.2749813E-722.4991631
GGGGCTA602.4635132E-422.4991636
CCCGCAT855.017817E-621.1756832
GCCCCTA754.592553E-520.99921627
TCGAGTG754.592553E-520.99921613
CTCGTAT654.2314196E-420.76845439
GTATGCC654.2314196E-420.76845442
ATCTACG550.003932157520.4537832
Produced by FastQC (version 0.11.2)